anti– phospho-pear1 ser891 antibody (GL Biochem)

GL Biochem anti– phospho-pear1 ser891 antibody
Anti– Phospho Pear1 Ser891 Antibody, supplied by GL Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pear1/10__1172_slash_jci177357-222-2-12?v=GL+Biochem
Average 90 stars, based on 1 article reviews

anti– phospho-pear1 ser891 antibody - by Bioz Stars, 2026-08

90/100 stars

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antibody against pear1 (Abbkine Inc)

Abbkine Inc antibody against pear1

Figure 1. <t>PEAR1</t> expression during bovine MDSC differentiation. (A) Western blotting analysis of differentiated MDSCs, showing the expression of PEAR1, MYH3, and MyoG at various stages. D0 indicates undifferentiated MDSCs and D1 to D5 indicate MDSCs on days 1 to 5, respectively, after initial exposure to differentiation medium. (B-D) Quantification of PEAR1, MYH3, and MyoG protein expression at various differentiation stages; compared to the D0 group (n = 3), *P < 0.05, **P < 0.01, **P < 0.001, NS: no significant difference. (E) Immunofluorescent staining for PEAR1 in MSDCs on differentiation day 0 (D0), 1 (D1), and 2 (D2). The green and blue signals represent PEAR1 and DAPI-stained nuclei, respectively (magnification, 200×).

Antibody Against Pear1, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pear1/pm31096840-152-7-10?v=Abbkine+Inc
Average 86 stars, based on 1 article reviews

antibody against pear1 - by Bioz Stars, 2026-08

86/100 stars

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bv421 mouse anti human pear1 (BD Biosciences)

N/A

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bv605 mouse anti human pear1 (BD Biosciences)

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anti-pear1 (aa 23-97) polyclonal antibody (Creative BioMart)

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When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells.Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.http://www.creative-diagnostics.com/Anti-PEAR1-PAb-210757-147.htm

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rabbit anti-human pear1 polyclonal antibody (Cusabio)

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Rabbit anti-Human PEAR1 Polyclonal Antibody

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bv650 mouse anti human pear1 (BD Biosciences)

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sheep polyclonal anti-mouse pear1 (Angio-Proteomie)

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anti-pear1 antibody (Boster Bio)

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Boster Bio Anti-PEAR1 Antibody (Catalog # A09593). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

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anti-pear1 antibody (St Johns Laboratory)

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Unconjugated Rabbit polyclonal to PEAR1 Conjugation note: Unconjugated Application note: WB, ELISA Reactivity note: Human, Mouse

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anti-pear1 (c-terminal) polyclonal antibody (Creative BioMart)

N/A

PEAR1 is a platelet receptor that signals upon the formation of platelet-platelet contacts independent of platelet activation and secondary to platelet aggregation (Nanda et al., 2005 [PubMed 15851471]).[supplied by OMIM, Mar 2008]Shipped at 4°C. Upon

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Figure 1. PEAR1 expression during bovine MDSC differentiation. (A) Western blotting analysis of differentiated MDSCs, showing the expression of PEAR1, MYH3, and MyoG at various stages. D0 indicates undifferentiated MDSCs and D1 to D5 indicate MDSCs on days 1 to 5, respectively, after initial exposure to differentiation medium. (B-D) Quantification of PEAR1, MYH3, and MyoG protein expression at various differentiation stages; compared to the D0 group (n = 3), *P < 0.05, **P < 0.01, **P < 0.001, NS: no significant difference. (E) Immunofluorescent staining for PEAR1 in MSDCs on differentiation day 0 (D0), 1 (D1), and 2 (D2). The green and blue signals represent PEAR1 and DAPI-stained nuclei, respectively (magnification, 200×).

Journal: Cell adhesion & migration

Article Title: Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase.

doi: 10.1080/19336918.2019.1619434

Figure Lengend Snippet: Figure 1. PEAR1 expression during bovine MDSC differentiation. (A) Western blotting analysis of differentiated MDSCs, showing the expression of PEAR1, MYH3, and MyoG at various stages. D0 indicates undifferentiated MDSCs and D1 to D5 indicate MDSCs on days 1 to 5, respectively, after initial exposure to differentiation medium. (B-D) Quantification of PEAR1, MYH3, and MyoG protein expression at various differentiation stages; compared to the D0 group (n = 3), *P < 0.05, **P < 0.01, **P < 0.001, NS: no significant difference. (E) Immunofluorescent staining for PEAR1 in MSDCs on differentiation day 0 (D0), 1 (D1), and 2 (D2). The green and blue signals represent PEAR1 and DAPI-stained nuclei, respectively (magnification, 200×).

Article Snippet: The membrane was incubated with a primary antibody against PEAR1 (Abbkine), MYOG (Santa Cruz), GAPDH (Proteintech), MYH3 (Santa Cruz), p-FAK (Bioss), FAK(Bioss), p-paxillin (Bioss), paxillin (Bioss), vinculin (Bioss), or ITGB1 (Bioss), followed by addition of a secondary horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG antibody (Bioss).

Techniques: Expressing, Western Blot, Staining

Figure 2. PEAR1 affects cell migration and interacts with ITGB1. (A-B) Cell scratch test. Ctrl is a sample transfected with the empty vector, VPR-P2 is a sample transfected with PEAR1 overexpression vector, dCas9-P3 is the suppression vector; ‘0 h’ indicates cells before migration, and ‘48 h’ indicates cell migration for 48 h under differentiation conditions. (C-D) Quantification of cell migration assay after overexpression or suppression of PEAR1. **P < 0.01, compared to the 0 h group (n = 3). (E) Immunofluorescent staining for PEAR1, microfilaments, and nuclei under differentiation conditions. The red signal represents PEAR1, the green signal represents microfilaments, and the blue signal represents nuclei stained with DAPI. (F) Immunoprecipitation with anti-PEAR1 antibodies followed by western blotting with anti-ITGB1 antibodies. InPut is a positive control, IgG is a negative control, IP is the target. (G) Immunoprecipitation with anti-ITGB1 antibodies followed by western blotting with anti-PEAR1 antibodies.

Journal: Cell adhesion & migration

Article Title: Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase.

doi: 10.1080/19336918.2019.1619434

Figure Lengend Snippet: Figure 2. PEAR1 affects cell migration and interacts with ITGB1. (A-B) Cell scratch test. Ctrl is a sample transfected with the empty vector, VPR-P2 is a sample transfected with PEAR1 overexpression vector, dCas9-P3 is the suppression vector; ‘0 h’ indicates cells before migration, and ‘48 h’ indicates cell migration for 48 h under differentiation conditions. (C-D) Quantification of cell migration assay after overexpression or suppression of PEAR1. **P < 0.01, compared to the 0 h group (n = 3). (E) Immunofluorescent staining for PEAR1, microfilaments, and nuclei under differentiation conditions. The red signal represents PEAR1, the green signal represents microfilaments, and the blue signal represents nuclei stained with DAPI. (F) Immunoprecipitation with anti-PEAR1 antibodies followed by western blotting with anti-ITGB1 antibodies. InPut is a positive control, IgG is a negative control, IP is the target. (G) Immunoprecipitation with anti-ITGB1 antibodies followed by western blotting with anti-PEAR1 antibodies.

Techniques: Migration, Transfection, Plasmid Preparation, Over Expression, Cell Migration Assay, Staining, Immunoprecipitation, Western Blot, Positive Control, Negative Control

Figure 3. PEAR1 affected the focal adhesions and bovine MDSC differentiation. (A) PEAR1 overexpression or suppression in bovine MDSCs. Ctrl is a sample transfected with the empty vector, VPR-P2 is a sample transfected with PEAR1 overexpression vector, dCas9-P3 is the suppression vector; (B-F) Quantification of the results shown in A. (G) Immunofluorescentce staining of Desmin in bovine MDSCs following PEAR1 overexpression or interference (magnification, 200×). (H) Quantification of myotube fusion rate based on desmin staining and visualization of total nuclei with DAPI. *P < 0.05, **P < 0.01, **P < 0.001, NS: no significant difference, compared to the control group (n = 3).

Journal: Cell adhesion & migration

Article Title: Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase.

doi: 10.1080/19336918.2019.1619434

Figure Lengend Snippet: Figure 3. PEAR1 affected the focal adhesions and bovine MDSC differentiation. (A) PEAR1 overexpression or suppression in bovine MDSCs. Ctrl is a sample transfected with the empty vector, VPR-P2 is a sample transfected with PEAR1 overexpression vector, dCas9-P3 is the suppression vector; (B-F) Quantification of the results shown in A. (G) Immunofluorescentce staining of Desmin in bovine MDSCs following PEAR1 overexpression or interference (magnification, 200×). (H) Quantification of myotube fusion rate based on desmin staining and visualization of total nuclei with DAPI. *P < 0.05, **P < 0.01, **P < 0.001, NS: no significant difference, compared to the control group (n = 3).

Techniques: Over Expression, Transfection, Plasmid Preparation, Staining, Control

Figure 4. FAK inhibition affects bovine MDSC differentiation. (A) Inhibits FAK activity, overexpresses PEAR1, overexpresses PEAR1 and inhibits FAK activity expression in bovine MDSCs for differentiation 48 h after treatment. (B-F) Quantification of the results shown in A . (G) Immunofluorescent staining for desmin in MDSCs following FAK inhibition, PEAR1 overexpression, and PEAR1 overexpression in the presence of the FAK inhibitor (magnification, 200×). (H) Quantification of the myotube fusion rate based on desmin staining and visualization of total nuclei with DAPI in G. *P < 0.05, **P < 0.01, **P < 0.001, NS: no significant difference.

Journal: Cell adhesion & migration

Article Title: Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase.

doi: 10.1080/19336918.2019.1619434

Figure Lengend Snippet: Figure 4. FAK inhibition affects bovine MDSC differentiation. (A) Inhibits FAK activity, overexpresses PEAR1, overexpresses PEAR1 and inhibits FAK activity expression in bovine MDSCs for differentiation 48 h after treatment. (B-F) Quantification of the results shown in A . (G) Immunofluorescent staining for desmin in MDSCs following FAK inhibition, PEAR1 overexpression, and PEAR1 overexpression in the presence of the FAK inhibitor (magnification, 200×). (H) Quantification of the myotube fusion rate based on desmin staining and visualization of total nuclei with DAPI in G. *P < 0.05, **P < 0.01, **P < 0.001, NS: no significant difference.

Techniques: Inhibition, Activity Assay, Expressing, Staining, Over Expression

Figure 5. ITGB1 silence affects bovine MDSC differentiation. (A) ITGB1 suppression, PEAR1 overexpression, PEAR1 overexpression and ITGB1 suppression in MDSCs. (B-G) Quantification of the results shown in A. (H) Immunofluorescent staining of desmin in bovine MDSCs following ITGB1 suppression, PEAR1 overexpression, PEAR1 overexpression and ITGB1 suppression (magnification, 200×). (I) Quantification of myotube fusion rate based on desmin staining and quantification of total DNA (DAPI) from H. (J) Immunofluorescent staining for PEAR1, microfilaments, and nuclei in MSDCs on day 2 of differentiation. The red, green, and blue signals represent PEAR1, microfilament, and nuclei stained by DAPI, respectively. *P < 0.05, **P < 0.01, **P < 0.001, NS: no significant difference.

Journal: Cell adhesion & migration

Article Title: Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase.

doi: 10.1080/19336918.2019.1619434

Figure Lengend Snippet: Figure 5. ITGB1 silence affects bovine MDSC differentiation. (A) ITGB1 suppression, PEAR1 overexpression, PEAR1 overexpression and ITGB1 suppression in MDSCs. (B-G) Quantification of the results shown in A. (H) Immunofluorescent staining of desmin in bovine MDSCs following ITGB1 suppression, PEAR1 overexpression, PEAR1 overexpression and ITGB1 suppression (magnification, 200×). (I) Quantification of myotube fusion rate based on desmin staining and quantification of total DNA (DAPI) from H. (J) Immunofluorescent staining for PEAR1, microfilaments, and nuclei in MSDCs on day 2 of differentiation. The red, green, and blue signals represent PEAR1, microfilament, and nuclei stained by DAPI, respectively. *P < 0.05, **P < 0.01, **P < 0.001, NS: no significant difference.

Techniques: Over Expression, Staining

Figure 6. Scheme of PEAR1-mediated regulation of bovine MDSC migration and differentiation. In early stages of cell differentiation, the expression of PEAR1 increases at the cellular surface. Interactions between PEAR1 and its ligand, ITGB1, on adjacent cell membrane induce the formation of a complex. Activated ITGB1 receptor recruits focal adhesion proteins and a complex is formed, by FAK, paxillin, and vinculin, which in turn affects cell migration and differentiation.

Journal: Cell adhesion & migration

Article Title: Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase.

doi: 10.1080/19336918.2019.1619434

Figure Lengend Snippet: Figure 6. Scheme of PEAR1-mediated regulation of bovine MDSC migration and differentiation. In early stages of cell differentiation, the expression of PEAR1 increases at the cellular surface. Interactions between PEAR1 and its ligand, ITGB1, on adjacent cell membrane induce the formation of a complex. Activated ITGB1 receptor recruits focal adhesion proteins and a complex is formed, by FAK, paxillin, and vinculin, which in turn affects cell migration and differentiation.

Techniques: Migration, Cell Differentiation, Expressing, Membrane

anti pear1 Search Results

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